Role of plasmodium falciparum protein GEXP07 in Maurer’s Cleft morphology, Knob architecture, and P. Falciparum EMP1 trafficking

E McHugh; OMS Carmo; A Blanch; O Looker; B Liu; S Tiash; D Andrew; S Batinovic; AJY Low; HJ Cho; P McMillan; L Tilley; MWA Dixon

Journal article

Role of plasmodium falciparum protein GEXP07 in Maurer’s Cleft morphology, Knob architecture, and P. Falciparum EMP1 trafficking

E McHugh, OMS Carmo, A Blanch, O Looker, B Liu, S Tiash, D Andrew, S Batinovic, AJY Low, HJ Cho, P McMillan, L Tilley, MWA Dixon

Mbio | AMER SOC MICROBIOLOGY | Published : 2020

DOI: 10.1128/mBio.03320-19

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Abstract

The malaria parasite Plasmodium falciparum traffics the virulence protein P. falciparum erythrocyte membrane protein 1 (PfEMP1) to the surface of infected red blood cells (RBCs) via membranous organelles, known as the Maurer’s clefts. We developed a method for efficient enrichment of Maurer’s clefts and profiled the protein composition of this trafficking organelle. We identified 13 previously uncharacterized or poorly characterized Maurer’s cleft proteins. We generated transfectants expressing green fluorescent protein (GFP) fusions of 7 proteins and confirmed their Maurer’s cleft location. Using co-immunoprecipitation and mass spectrometry, we generated an interaction map of proteins at th..

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University of Melbourne Researchers

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Awarded by Australian Research Council

Funding Acknowledgements

L.T. is a Georgina Sweet, Australian Research Council Laureate Fellow (LE150100011) (http://www.arc.gov.au).M.W.A.D.and L.T. thank the Australian Research Council (DP110100624) (http://www.arc.gov.au) and the National Health and Medical Research Council (1098992) (https://www.nhmrc.gov.au) for funding this work. M.W.A.D. was supported by a National Health and Medical Research Council Training fellowship (602541).